2008—2012年山东省鸡传染性支气管炎病毒遗传演化分析和致病性研究

doi:10.11843/j.issn.0366-6964.2014.05.017

Abstract

Abstract:

The aim of this study was to further determine the evolution and epidemiological situation of infectious bronchitis virus (IBV) in Shandong Province.The S1，N and M genes of twenty IBV strains isolated from diseased chickens in Shandong Province between 2008 and 2012 were sequenced.Besides，the pathogenicity of three isolates was evaluated in 18-day-old specific-pathogen-free (SPF) chickens.Phylogenetic analysis based on the S1 genes (1 611 bp) revealed that 20 IBV isolates and reference strains were grouped into four distinct clusters (genotypes).Eighteen IBV isolates belonged to the QX genotype.Two isolates，CK/CH/SD09/005 and SDIB781/2012 were typed into a separate cluster—genotype Ⅳ and showed only 65.1%-67.1% nucleotide identities to IBVs of the other three genotypes.CK/CH/SD09/005 and SDIB781/2012 showed nucleotide identities of 97.8% in S1 gene while they shared only 86.7% and 87.9% nucleotide identities in the N and M genes，respectively.SDIB781/2012 had nucleotide identities of 100% with SDIB764/2012 and SDIB702/2012 (QX-like ) in N and M genes，respectively，which indicated genetic recombinations between strains of QX-like and gentotype Ⅳ.In the challenge test，SDZB0808，SDIB821/2012 and CK/CH/SD09/005 resulted in deaths of 60%-70% in experimental infections of SPF chickens.Swollen speckled kidneys and distended ureters filled with uric acid were observed in dead birds.Besides，plenty of uric acid salt deposited on the surface of kidney，heart and other tissues of infected birds.There is no significant distinction in clinical and pathological changes of birds inoculated with three IBV strains.The results showed that the QX-like strains，the predominant type IBV，and a new genotype IBV had been co-circulating in Shandong Province in recent years.Both of them are Nephropathogenic strains.The recombinant events between IBV strains of QX and the new genotype emphasize the importance of a persistent survey of IBV field strains.

CLC Number:

S852.659.6

HU Bei-xia， YANG Shao-hua， XU Chuan-tian， ZHANG Wei， ZHANG Lin，HUANG Qing-hua， ZHANG Xiu-mei， HUANG Yan-yan， WEN Xin-tian. Phylogenetic Analysis and Pathogenicity of Infectious Bronchitis Viruses Isolated in Shandong Province during 2008-2012[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 795-801.

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Phylogenetic Analysis and Pathogenicity of Infectious Bronchitis Viruses Isolated in Shandong Province during 2008-2012

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